Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Factor B ELISA Kit (ab137973)

Key features and details

  • Sensitivity: 0.8 ng/ml
  • Range: 4.375 ng/ml - 140 ng/ml
  • Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Factor B ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.3%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.8 ng/ml

  • Range

    4.375 ng/ml - 140 ng/ml

  • Recovery

    98 %

  • Assay time

    4h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Factor B Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Factor B in plasma, serum, saliva, milk, and cell culture supernatants.


    A Factor B specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor B specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor B captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Factor B Antibody 1 x 120µl
    Chromogen Substrate 1 x 7ml
    Factor B Microplate (12 x 8 well strips) 1 unit
    Factor B Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml

  • Research areas

  • Function

    Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

  • Involvement in disease

    Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 3 Sushi (CCP/SCR) domains.
    Contains 1 VWFA domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • AHUS4
    • B factor properdin
    • BF
    • BFD
    • C3 proaccelerator
    • C3 proactivator
    • C3/C5 convertase
    • CFAB
    • CFAB_HUMAN
    • CFB
    • Complement Factor B
    • Complement factor B Bb fragment
    • FB
    • FBI12
    • GBG
    • Glycine rich beta glycoprotein
    • Glycine-rich beta glycoprotein
    • H2 Bf
    • H2BF
    • PBF2
    • Properdin factor B
    see all

  • Database links

    Images

    • ELISA: Factor B Human ELISA Kit (ab137973)
      ELISA: Factor B Human ELISA Kit (ab137973)

      Standard curve with background signal subtracted (duplicates; +/- SD).

    • ELISA: Factor B Human ELISA Kit (ab137973)
      ELISA: Factor B Human ELISA Kit (ab137973)

      Factor B measured in culture supernatants (dilution 1/1-1/30; duplicates +/- SD).

    • ELISA: Factor B Human ELISA Kit (ab137973)
      ELISA: Factor B Human ELISA Kit (ab137973)

      Factor B in biological fluids (duplicates +/- SD). Tested dilution ranges: human plasma and serum at 1/1000-1/30000, others at 1/1-1/300.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab137973

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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