Human ERAP2 ELISA Kit (ab277422)
Key features and details
- Sensitivity: 1.23 ng/ml
- Range: 1.229 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ERAP2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.23 ng/ml -
Range
1.229 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94.06 68% - 124% Plasma 82.22 68% - 97% Cell culture media 108 72% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human ERAP2 ELISA Kit (ab277422) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ERAP2 in serum, plasma and cell culture supernatants
This assay employs an antibody specific for Human ERAP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ERAP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ERAP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ERAP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human ERAP2 Antibody 2 vials Anti-Human ERAP2 coated Microplate 1 unit Human ERAP2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
ERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys. -
Cellular localization
Endoplasmic reticulum membrane; Single-pass type II membrane protein. -
Alternative names
- Endoplasmic reticulum aminopeptidase 2
- L RAP
- Leukocyte derived arginine aminopeptidase
- LRAP
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Database links
- Entrez Gene: 64167 Human
- Omim: 609497 Human
- SwissProt: Q6P179 Human
- Unigene: 482910 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.