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Immunology Innate Immunity Chemokines Beta Chemokines (CC)

Human Eotaxin-2 Antibody Pair - BSA and Azide free (ab244035)

Human Eotaxin-2 Antibody Pair - BSA and Azide free (ab244035)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human Eotaxin-2 Antibody Pair - BSA and Azide free
    See all Eotaxin 2 kits
  • Assay type

    ELISA set
  • Range

    78.13 pg/ml - 5000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Eotaxin-2. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab226898), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Eotaxin-2 Capture Antibody (unconjugated) 1 x 100µg
    Human Eotaxin-2 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
  • Function

    Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
  • Tissue specificity

    Activated monocytes and activated T lymphocytes.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession O00175 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • C C motif chemokine 24
    • C-C motif chemokine 24
    • CCL24
    • CCL24_HUMAN
    • Chemokine CC Motif Ligand 24
    • CK beta 6
    • CK-beta-6
    • Ckb6
    • Eosinophil chemotactic protein 2
    • Eotaxin-2
    • MPIF 2
    • MPIF-2
    • MPIF2
    • Myeloid progenitor inhibitory factor 2
    • SCYA24
    • Small inducible cytokine A24
    • Small inducible cytokine subfamily A (Cys-Cys) member 24
    • Small-inducible cytokine A24
    see all
  • Database links

    • Entrez Gene: 6369 Human
    • Omim: 602495 Human
    • SwissProt: O00175 Human
    • Unigene: 247838 Human

    Images

    • Sandwich ELISA - Human Eotaxin-2 Antibody Pair - BSA and Azide free (ab244035)
      Sandwich ELISA - Human Eotaxin-2 Antibody Pair - BSA and Azide free (ab244035)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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