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Signal Transduction Cytoskeleton / ECM Basal Lamina

Human Entactin ELISA Kit (NID1) (ab113338)

Human Entactin ELISA Kit (NID1) (ab113338)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Sensitivity: 0.1 ng/ml
  • Range: 0.123 ng/ml - 30 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human Entactin 2 vials
    Entactin Microplate (12 x 8 strips) 1 unit
    Recombinant Human Entactin Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Adhesion molecules ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Developmental proteins ELISA kits
  • Function

    Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
  • Sequence similarities

    Contains 6 EGF-like domains.
    Contains 4 LDL-receptor class B repeats.
    Contains 1 NIDO domain.
    Contains 1 nidogen G2 beta-barrel domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Target information above from: UniProt accession P14543 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Enactin
    • Ent
    • Entactin
    • Entactin 1
    • NID
    • NID 1
    • NID-1
    • Nid1
    • NID1_HUMAN
    • Nidogen
    • Nidogen (enactin)
    • Nidogen 1
    • Nidogen-1
    see all
  • Database links

    • Entrez Gene: 4811 Human
    • Entrez Gene: 4811 Human
    • Omim: 131390 Human
    • SwissProt: P14543 Human
    • SwissProt: P14543 Human
    • Unigene: 356624 Human

    Images

    • Representative Standard Curve
      Representative Standard Curve

      Representative Standard Curve using ab113338

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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