Human Endocan ELISA Kit (ESM-1) (ab278119)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.12 ng/ml
- Range: 0.19 ng/ml - 12 ng/ml
- Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Endocan ELISA Kit (ESM-1)
See all ESM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 4.2% Inter-assay Sample n Mean SD CV% Plasma 3 3.7% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.12 ng/ml -
Range
0.19 ng/ml - 12 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 93% - 95% Cell culture media 86 81% - 93% Hep Plasma 93 91% - 96% EDTA Plasma 118 117% - 120% Cit plasma 89 84% - 92% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Endocan ELISA kit (ab278119) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Endocan protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Endocan with 0.12 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Endocan, or Endothelial cell-specific molecule 1 (ESM-1) is a protein encoded by the ESM1 gene. Endocan is expressed as a 184- amino acid protein containing a 19 -amino acid signal peptide, and the secreted mature chain contains chondroitin sulfate and dermatan sulfate. Endocan is mainly expressed in the endothelial cells in lung and kidney and is regulated by cytokines. Endocan is involved in angiogenesis and promotes angiogenic sprouting. Endocan is Induced by TNF and IL1B/interleukin-1 beta, but not IL-4.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Endocan (ESM-1) Capture Antibody 1 x 600µl 10X Human Endocan (ESM-1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Endocan (ESM-1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May have potent implications in lung endothelial cell-leukocyte interactions. -
Tissue specificity
Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney. -
Sequence similarities
Contains 1 IGFBP N-terminal domain. -
Post-translational
modificationsMay contain intrachain disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Endocan
- Endothelial cell specific molecule 1
- Endothelial cell-specific molecule 1
see all -
Database links
- Entrez Gene: 11082 Human
- Omim: 601521 Human
- SwissProt: Q9NQ30 Human
- Unigene: 129944 Human
Images
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Recombinant Antibody benefits
To learn more about the advantages of recombinant antibodies see here.
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SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Endocan standard curve in Sample Diluent NS.The Endocan standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Endocan in human plasma (EDTA) samples.The concentrations of Endocan were measured in duplicates, interpolated from the Endocan standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endocan concentration was determined to be 2.3 ng/mL in plasma (EDTA).
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Serum from eight individual healthy female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endocan concentration was determined to be 0.45 ng/mL with a range of 0.29 – 0.71 ng/mL where detected.