Human Egr1 ELISA Kit (ab277410)
Key features and details
- Sensitivity: 51 pg/ml
- Range: 51.2 pg/ml - 12500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Egr1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
51 pg/ml -
Range
51.2 pg/ml - 12500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 134.1 118% - 140% Plasma 109.4 94% - 130% Cell culture media 114.3 92% - 144% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Egr1 ELISA Kit (ab277410) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Egr1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Egr1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Egr1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Egr1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Egr1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human Egr1 Antibody 2 vials Anti-Human Egr1 coated Microplate 1 unit Human Egr1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. -
Sequence similarities
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- AT225
- Early growth response 1
- Early growth response protein 1
see all -
Database links
- Entrez Gene: 1958 Human
- Omim: 128990 Human
- SwissProt: P18146 Human
- Unigene: 326035 Human
- Unigene: 708393 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.