Human DPP4 (CD26) ELISA Kit (ab252365)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12.82 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DPP4 (CD26) ELISA Kit
See all DPP4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 5.6% Inter-assay Sample n Mean SD CV% Serum 3 3.1% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12.82 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 88% - 106% Saliva 98 91% - 105% Urine 105 102% - 109% Serum 102 100% - 104% Hep Plasma 101 96% - 106% EDTA Plasma 101 95% - 111% Cit plasma 103 103% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human CD26 ELISA Kit (ab252365) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD26 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD26 with 12.82 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CD26, also known as DPP4 (Dipeptidyl Peptidase IV), is a glycoprotein receptor essential for T cell receptor-mediated T cell activation. CD26 is a serine protease that cleaves Xaa-Pro dipeptides from the N terminus of oligo-and polypeptides. Involved in a variety of cellular processes, CD26 is a therapeutic target in clinical trials for type 2 diabetes.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human DPP4 (CD26) Capture Antibody 1 x 600µl 10X Human DPP4 (CD26) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human DPP4 (CD26) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. -
Tissue specificity
Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. -
Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily. -
Domain
The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. -
Post-translational
modificationsThe soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. -
Cellular localization
Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid. - Information by UniProt
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Alternative names
- CD26 antigen
- ADA-binding protein
- ADABP
see all -
Database links
- Entrez Gene: 1803 Human
- Omim: 102720 Human
- SwissProt: P27487 Human
- Unigene: 368912 Human
Images
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Other - Human CD26 ELISA Kit (ab252365)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CD26 Standard Curve ComparisonStandard Curve comparison between human CD26 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human CD26 standard curve in Sample Diluent NS.The CD26 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD26 in human serum and plasma samples.The concentrations of CD26 were measured in duplicates, interpolated from the CD26 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 100, plasma (citrate) 1: 100, plasma (heparin) 1: 100, and plasma (EDTA) 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 570.0 ng/mL in serum, 470.9 ng/mL in plasma (citrate), 514.4 ng/ml in plasma (heparin), and 524.8 ng/ml in plasma (EDTA).
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Interpolated concentrations of native CD26 in human male urine, female saliva, PBMC cell culture supernatant stimulated for 46 hours with 1.5% PHA-M, and HepG2 cell culture supernatant samples.The concentrations of CD26 were measured in duplicates, interpolated from the CD26 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 12.5%, saliva 50%, PBMC supernatant 100%, and HepG2 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 36.1 ng/mL in urine, 13.5 ng/ml in saliva, 1.0 ng/ml in stimulated PBMC supernatant, and 5.8 ng/ml in HepG2 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 300.8 ng/mL with a range of 200.7 – 421.5 ng/mL.
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Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (ab252365)To learn more about the advantages of recombinant antibodies see here.
