Human DMP1 ELISA Kit (ab246530)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 22.58 pg/ml
- Range: 171.875 pg/ml - 11000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DMP1 ELISA Kit
See all DMP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 6.6% Inter-assay Sample n Mean SD CV% Supernatant 3 6.7% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
22.58 pg/ml -
Range
171.875 pg/ml - 11000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 93% - 120% Serum 83 82% - 84% Cit plasma 84 83% - 86% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human DMP1 ELISA Kit (ab246530) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DMP1 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DMP1 with 22.58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human DMP1 Capture Antibody 1 x 600µl 10X Human DMP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human DMP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
DMP1 is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. It is critical for proper mineralization of bone and dentin, and is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. DMP1 may also have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts the unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. -
Cellular localization
Nucleus. Cytoplasm. Secreted; extracellular space; extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralizated matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus. -
Alternative names
- ARHP
- ARHR
- AV020965
see all -
Database links
- Entrez Gene: 1758 Human
- Entrez Gene: 1758 Human
- Omim: 600980 Human
- SwissProt: Q13316 Human
- SwissProt: Q13316 Human
Images
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Other - Human DMP1 ELISA Kit (ab246530)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human DMP1 standard curve in Sample Diluent 75BS.The DMP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human DMP1 standard curve in Sample Diluent NS.The DMP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked DMP1 in human serum, plasma (citrate), and PBMC cell culture supernatant samples.The concentrations of DMP1 were measured in duplicates, interpolated from the DMP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and PBMC supernatant 50%. The interpolated values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human DMP1 ELISA Kit (ab246530)To learn more about the advantages of recombinant antibodies see here.
