Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human DKK1 knockout HeLa cell pellet (ab279182)

Overview

  • Product name

    Human DKK1 knockout HeLa cell pellet
    See all DKK1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 223 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

  • Tissue specificity

    Placenta.

  • Sequence similarities

    Belongs to the dickkopf family.

  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Dickkopf 1
    • Dickkopf 1 homolog
    • Dickkopf 1 like
    • Dickkopf homolog 1
    • Dickkopf like protein 1
    • Dickkopf related protein 1
    • Dickkopf WNT signaling pathway inhibitor 1
    • Dickkopf-1
    • Dickkopf-related protein 1
    • DKK 1
    • Dkk-1
    • Dkk1
    • DKK1_HUMAN
    • hDkk 1
    • hDkk-1
    • SK
    see all

Properties

Images

  • Sanger Sequencing - Human DKK1 knockout HeLa cell pellet (ab279182)
    Sanger Sequencing - Human DKK1 knockout HeLa cell pellet (ab279182)

    Allele-1: 223 bp deletion in exon 1

     

  • Sanger Sequencing - Human DKK1 knockout HeLa cell pellet (ab279182)
    Sanger Sequencing - Human DKK1 knockout HeLa cell pellet (ab279182)

    Allele-2: Insertion of the selection cassette in exon 1

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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