Human DIAPH1 knockout HCT116 cell line (ab273727)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Colon -
Cell type
epithelial -
Disease
Carcinoma -
Gender
Male -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Purity
Immunogen affinity purified -
Research areas
Images
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Western blot - Human DIAPH1 knockout HCT116 cell line (ab273727)All lanes : Anti-DIAPH1 antibody [EPR7948] (ab129167) at 1/1000 dilution
Lane 1 : Wild-type HCT116 cell lysate
Lane 2 : DIAPH1 knockout HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab129167 observed at 155 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab129167 was shown to react with DIAPH1 in wild-type HCT 116 cells in western blot with loss of signal observed in DIAPH1 knockout cell line ab273727 (DIAPH1 knockout cell lysate ab275252). Wild-type and DIAPH1 knockout HCT 116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab129167 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human DIAPH1 knockout HCT116 cell line (ab273727)Allele-1: 2 bp deletion in exon 1 .
