Human Cyclin E1 ELISA Kit (ab231929)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.2 ng/ml
- Range: 3.91 ng/ml - 250 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cyclin E1 ELISA Kit
See all Cyclin E1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 5 2.1% Inter-assay Sample n Mean SD CV% Cell extract 3 12.4% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 ng/ml -
Range
3.91 ng/ml - 250 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 104 98% - 108% Tissue Extracts 101 98% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cyclin E1 ELISA Kit (ab231929) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin E1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin E1 with 1.2 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cyclin E1 Capture Antibody 1 x 600µl 10X Human Cyclin E1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Cyclin E1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Essential for the control of the cell cycle at the G1/S (start) transition. -
Tissue specificity
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
Sequence similarities
Belongs to the cyclin family. Cyclin E subfamily. -
Post-translational
modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- CCNE
- Ccne1
- CCNE1_HUMAN
see all -
Database links
- Entrez Gene: 898 Human
- Omim: 123837 Human
- SwissProt: P24864 Human
- Unigene: 244723 Human
Images
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Other - Human Cyclin E1 ELISA Kit (ab231929)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cyclin E1 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cyclin E1 in JAR cell extract based on a 1,000 µg/mL extract loadThe concentrations of Cyclin E1 were measured in duplicate and interpolated from the Cyclin E1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin E1 concentration was determined to be 163 ng/mL in JAR cell extract.
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Interpolated concentrations of native Cyclin E1 in HeLa cell extract based on a 1,500 µg/mL extract loadThe concentrations of Cyclin E1 were measured in duplicate and interpolated from the Cyclin E1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin E1 concentration was determined to be 54 ng/mL in HeLa cell extract.
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Sandwich ELISA - Human Cyclin E1 ELISA Kit (ab231929)To learn more about the advantages of recombinant antibodies see here.
