Human CXCL7 / PBP Matched Antibody Pair Kit (ab219537)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 3.9 pg/ml - 250 pg/ml
Overview
-
Product name
Human CXCL7 / PBP Matched Antibody Pair Kit
See all CXCL7/PBP kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.75 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human CXCL7 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human CXCL7.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216171. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human CXCL7 Capture Antibody 2 x 50µg 1 x 50µg Human CXCL7 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human CXCL7 Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- B TG1
- Beta TG
- Beta thromboglobulin
see all -
Database links
- Entrez Gene: 5473 Human
- Omim: 121010 Human
- SwissProt: P02775 Human
- Unigene: 2164 Human
Images
-
Human CXCL7 Standard Curve
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Human CXCL7 / PBP Matched Antibody Pair Kit (ab219537)To learn more about the advantages of recombinant antibodies see here.
