Human Complement Factor P ELISA Kit (ab222864)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.156 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement Factor P ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 1 20 5.1% Plasma 2 20 4.8% Plasma 3 20 5% Inter-assay Sample n Mean SD CV% Plasma 1 20 9.9% Plasma 2 20 9.7% Plasma 3 20 10.2% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.156 ng/ml - 10 ng/ml -
Recovery
96 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Rabbit, Cow, Dog, Pig -
Product overview
The Human Complement Factor P (CFP) ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab222864) is designed for detection of Complement Factor P in human plasma, serum, urine, saliva, CSF, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Complement Factor P in approximately 4 hours. A polyclonal antibody specific for human Complement Factor P has been pre-coated onto a 96-well microplate with removable strips. Complement Factor P in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human Complement Factor P, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Complement factor P (CFP), also known as properdin, is a plasma glycoprotein that positively regulates the alternative complement pathway convertases (C3bBb) of the innate immune system. CFP consists of 442-amino acids with a molecular weight of 53 kDa and is composed of multiple identical protein subunits. The subunits bind to each other in a head to tail manner to form cyclic dimers, trimers, tetramers, pentamers, and higher cyclic oligomers. It is mainly produced by neutrophils but also by monocytes, T cells, and bone marrow progenitor cells. CFP binds to many microbial surfaces and apoptotic cells. Complement factor P also stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement Factor P 1 x 120µl Anti-Human Complement Factor P coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Human Complement Factor P Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Relevance
Factor P (Properdin) is a plasma protein that is active in the alternative complement pathway of the innate immune system. It is a positive regulatory factor that binds to many microbial surfaces to stabilize the C3b,Bb convertase. The C3b,Bb convertase then rapidly cleaves more C3 to C3b, which acts either as an opsonin or to reinitiate the pathway in an amplification loop that proceeds on the bacterial cell, but not on the host cell. In the alternative pathway, C3 is thus activated through factor B, factor D, and properdin P, under the control of factors I and H. Deficiency of properdin is associated in particular with a heightened susceptibility to Neisseria species. -
Cellular localization
Secreted -
Alternative names
- CFP
- Complement factor P
- PFC
- Properdin
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Database links
- Entrez Gene: 5199 Human
- Omim: 300383 Human
- SwissProt: P27918 Human
- Unigene: 53155 Human
Images
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Human Complement Factor P standard curve example
Example of human Complement Factor P standard curve in 1X Diluent M. The standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.