Human Complement C7 ELISA Kit (ab125964)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 2.5 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C7 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.2% Inter-assay Sample n Mean SD CV% Overall 10.1% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 ng/ml -
Range
2.5 ng/ml - 20 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Complement C7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C7 in Human plasma, serum, saliva, milk, urine and cell culture supernatants.
A Complement C7 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C7 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C7 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C7 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Complement C7 Microplate (12 x 8 well strips) 1 unit Complement C7 Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. -
Involvement in disease
Defects in C7 are a cause of complement component 7 deficiency (C7D) [MIM:610102]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
Sequence similarities
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 2 TSP type-1 domains. -
Post-translational
modificationsC7 has 28 disulfide bridges. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C7
- CO7_HUMAN
- complement component 7
- Complement component C7
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Database links
- Entrez Gene: 730 Human
- Omim: 217070 Human
- SwissProt: P10643 Human
- Unigene: 78065 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab125964