Human Complement C6 ELISA Kit (ab125965)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C6 ELISA Kit
See all C6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.7% Inter-assay Sample n Mean SD CV% Overall 10% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.391 ng/ml - 25 ng/ml -
Recovery
101 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Complement C6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C6 in Human plasma, serum, saliva, urine, milk, and cell culture supernatants.
A Complement C6 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C6 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C6 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C6 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Complement C6 Microplate (12 x 8 well strips) 1 unit Complement C6 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. -
Involvement in disease
Defects in C6 are the cause of complement component 6 deficiency (C6D) [MIM:612446]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
Sequence similarities
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 3 TSP type-1 domains. -
Post-translational
modificationsAll cysteine residues are assumed to be cross-linked to one another. Individual modules containing an even number of conserved cysteine residues are supposed to have disulfide linkages only within the same module. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AW111623
- C6
- CO6_HUMAN
see all -
Database links
- Entrez Gene: 729 Human
- Omim: 217050 Human
- SwissProt: P13671 Human
- Unigene: 481992 Human
Images
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125965 Complement C6 Human ELISA Kit (AssayPro)
Complement C6 measured in various biofluids showing quantity (microgram) per mL of sample tested
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125965 Complement C6 Human ELISA Kit (AssayPro)Colorimetric standard curve: mean of duplicates (+/-) with background readings subtracted