Human Complement C4 ELISA Kit (ab108825)
Key features and details
- Sensitivity: 41 pg/ml
- Range: 0.078 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C4 ELISA Kit
See all C4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.1% Inter-assay Sample n Mean SD CV% Overall 10% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 41 pg/ml -
Range
0.078 ng/ml - 20 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Complement C4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C4 levels in urine, milk, saliva, CSF and cell culture supernatants.
A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C4 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C4 Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Complement C4 Microplate (12 x 8 well strips) 1 unit Complement C4 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Acidic C4
- Acidic complement C4
- Basic C4
see all -
Database links
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Entrez Gene: 100293534 Human
- Entrez Gene: 721 Human
- Omim: 120810 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab108825
