Human CKMB ELISA Kit (ab193696)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.3 ng/ml - 80 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CKMB ELISA Kit
See all Creatine Kinase MM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3 ng/ml -
Range
0.3 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97.17 71% - 123% Plasma 87.57 78% - 108% Cell culture media 86.45 67% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's CKMB Human ELISA Kit (ab193696) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CKMB in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CKMB coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CKMB present in the samples. The wells are washed and biotinylated anti-Human CKMB antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CKMB bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent D 1 x 15ml 5X Assay Diluent B 1 x 15ml Biotinylated Human CKMB detection antibody (lyophilized) 2 vials Human CKMB Standards (lyophilized) 2 vials Pre-coated Human CKMB Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- CKM
- CKMM
- Creatine kinase M
see all -
Database links
- Entrez Gene: 1158 Human
- Omim: 123310 Human
- SwissProt: P06732 Human
- Unigene: 334347 Human
Images
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Example Standard Curve
