Human CEA ELISA Kit (ab264604)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.68 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CEA ELISA Kit
See all Carcino Embryonic Antigen CEA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 8 5% Inter-assay Sample n Mean SD CV% extract 3 5.4% -
Sample type
Serum, Cell culture extracts, Tissue Extracts, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24.68 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100 92% - 116% Cell culture extracts 108 95% - 122% Tissue Extracts 109 104% - 117% Cell culture media 93 84% - 99% Hep Plasma 101 90% - 110% EDTA Plasma 98 91% - 110% Cit plasma 105 91% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CEA ELISA Kit (ab264604) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CEA protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, cell culture extracts, cell lysate, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CEA with 24.68 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CEA is encoded by the CECAM5 gene which produces a cell adhesion molecule consisting of an Ig variable region-like amino-terminal domain followed by up to six Ig constant region-like domains anchored to the cell membrane by either a glycosylphosphatidylinositol (GPI) moiety or a proteinaceous transmembrane and cytoplasmic domain. CEA is normally expressed on epithelial cells and is found abundantly on the apical surface of the gastrointestinal epithelium, as well as the nasopharynx, lung, urogenital tract, and sweat glands. Under physiologic conditions, only low amounts of soluble CEA can be detected in serum. However, in many different cancers, CEA is highly up regulated.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CEA Capture Antibody 1 x 600µl 10X Human CEA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CEA Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. -
Tissue specificity
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. -
Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsComplex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Carcinoembryonic antigen
- Carcinoembryonic antigen-related cell adhesion molecule 5
- CD66e
see all -
Database links
- Entrez Gene: 1048 Human
- Omim: 114890 Human
- SwissProt: P06731 Human
- Unigene: 709196 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CEA standard curve comparisonStandard Curve comparison between human CEA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human CEA Simplestep ELISA comparisonStandard Curve comparison between ab264604 and ab183365 SimpleStep ELISA kits. ab264604 SimpleStep ELISA kit shows increased sensitivity.
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Example of human CEA standard curve in Sample Diluent NS.The CEA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CEA in human MCF7 and colon homogenate extract samples based on 250 and 125 µg/mL extract loads, respectively.The concentrations of CEA were measured in duplicate and interpolated from the CEA standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEA concentration was determined to be 3,584.91 pg/ml in MCF7 and 3,812.56 pg/mL in colon homogenate extract.
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Serum from ten individual healthy human female donors was measured in duplicate, one donor of which was non-detectible (ND) by this assay.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEA concentration was determined to be 1,027.57 pg/mL with a range of ND – 4,257.59 pg/mL.
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Sandwich ELISA - Human CEA ELISA Kit (ab264604)To learn more about the advantages of recombinant antibodies see here.
