Human CDCP1 ELISA Kit (ab253216)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 221 ng/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CDCP1 ELISA Kit
See all CDCP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 24 3.2% Inter-assay Sample n Mean SD CV% Extract 3 3.2% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
221 ng/ml -
Range
0.31 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 106 100% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CDCP1 ELISA Kit (ab253216) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CDCP1 protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CDCP1 with 221 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CDCP1 is a transmembrane protein involved in cell adhesion and signaling. Alongside the integrin receptors, CDCP1 helps control focal adhesion proteins, promoting anchorage or detachment of cells. Given its importance in cell motility, CDCP1 has been studied in the context of tumorigenesis where it has been found to be upregulated in many cancer types including breast cancer. It is currently thought to be a main driver of tumor invasion and metastasis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CDCP1 Capture Antibody 1 x 600µl 10X Human CDCP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Human CDCP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. -
Tissue specificity
Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. -
Sequence similarities
Contains 1 CUB domain. -
Post-translational
modificationsPhosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease. -
Cellular localization
Secreted and Cell membrane. Shedding may also lead to a soluble peptide. - Information by UniProt
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Alternative names
- 9030022E12Rik
- AA409659
- CD 318
see all -
Database links
- Entrez Gene: 64866 Human
- Omim: 611735 Human
- SwissProt: Q9H5V8 Human
- Unigene: 476093 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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. Example of human CDCP1 standard curve in 1X Cell Extraction Buffer PTR.The CDCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CDCP1 in HT-29 cell extracts based on a 12.5 µg/mL extract load.The concentrations of CDCP1 were measured in duplicate and interpolated from the CDCP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CDCP1 concentration was determined to be 8.36 ng/mL .
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Sandwich ELISA - Human CDCP1 ELISA Kit (ab253216)To learn more about the advantages of recombinant antibodies see here.
