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Human Cdc25C ELISA Kit (ab230944)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 19 pg/ml
  • Range: 78.1 pg/ml - 5000 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Cdc25C ELISA Kit
    See all Cdc25C kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    cell extract 8 3.8%
    Inter-assay
    Sample n Mean SD CV%
    cell extract 3 6.9%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    19 pg/ml

  • Range

    78.1 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 95 90% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Cdc25C ELISA Kit (ab230944) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cdc25C protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cdc25C with 19 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Cdc25C Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 11 x 66ml
    Human Cdc25C Detector Antibody (Lyophilized) 1 vial
    Human Cdc25C Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.

  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.

  • Developmental stage

    Expressed predominantly in G2 phase.

  • Post-translational
    modifications

    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Alternative names

    • CDC 25
    • Cdc 25C
    • CDC25
    • CDC25C
    • Cell division cycle 25 homolog C
    • Cell division cycle 25C
    • Cell division cycle 25C protein
    • Dual specificity phosphatase Cdc25C
    • M phase inducer phosphatase 3
    • M-phase inducer phosphatase 3
    • Mitosis inducer CDC25
    • MPIP3
    • MPIP3_HUMAN
    • Phosphotyrosine phosphatase
    • PPP1R60
    • protein phosphatase 1, regulatory subunit 60
    see all

  • Database links

    Images

    • Other - Human Cdc25C ELISA Kit (ab230944)
      Other - Human Cdc25C ELISA Kit (ab230944)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human Cdc25C standard curve in 1X Cell Extraction Buffer PTR.
      Example of human Cdc25C standard curve in 1X Cell Extraction Buffer PTR.

      The Cdc25C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Cdc25C in human Raji, K562, and Jurkat cell extract, sample based on a 500 µg/mL, 887 µg/mL, and 466µg/mL extract load, respectively.
      Interpolated concentrations of native Cdc25C in human Raji, K562, and Jurkat cell extract, sample based on a 500 µg/mL, 887 µg/mL, and 466µg/mL extract load, respectively.
    • Sandwich ELISA - Human Cdc25C ELISA Kit (ab230944)
      Sandwich ELISA - Human Cdc25C ELISA Kit (ab230944)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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