Human CD93 ELISA Kit (ab235636)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13.4 pg/ml
- Range: 70 pg/ml - 4500 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD93 ELISA Kit
See all CD93 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 6.4% Inter-assay Sample n Mean SD CV% Serum 3 5.5% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13.4 pg/ml -
Range
70 pg/ml - 4500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 94% - 105% Urine 103 98% - 109% Serum 107 100% - 119% Hep Plasma 100 99% - 100% EDTA Plasma 96 91% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human CD93 ELISA Kit (ab235636) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD93 protein in edta plasma, hep plasma, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD93 with 13.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD93 Capture Antibody 1 x 600µl 10X Human CD93 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CD93 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. -
Tissue specificity
Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 5 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- C1q receptor 1
- C1q/MBL/SPA receptor
- C1qR
see all -
Database links
- Entrez Gene: 22918 Human
- Omim: 120577 Human
- SwissProt: Q9NPY3 Human
- Unigene: 97199 Human
Images
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Other - Human CD93 ELISA Kit (ab235636)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CD93 Competitor Std Curve ComparisonStandard curve comparison between human CD93 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human CD93 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD93 in human serum and plasma samplesThe concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 2%, plasma (heparin) 2% and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 191.2 ng/mL in serum, 189.7 ng/mL in plasma (citrate), 166.8 ng/ml in plasma (heparin), and 255.0 ng/mL in plasma (EDTA).
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Interpolated concentrations of native CD93 in human urine and LPS-treated THP-1 supernatant samplesThe concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50% and LPS-treated THP-1 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 1.2 ng/mL in urine, and 6.3 ng/ml in treated THP-1 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 230.4 ng/mL with a range of 134.8 – 719.6 ng/mL.
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Sandwich ELISA - Human CD93 ELISA Kit (ab235636)To learn more about the advantages of recombinant antibodies see here.
