Human CD80 ELISA Kit (B7-1) (ab256392)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 31 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow, Human
Overview
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Product name
Human CD80 ELISA Kit (B7-1)
See all CD80 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.9% Inter-assay Sample n Mean SD CV% Serum 3 6.5% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
31 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 106% - 113% Serum 101 99% - 105% Cell culture media 97 95% - 100% Hep Plasma 92 88% - 98% EDTA Plasma 97 94% - 101% Cit plasma 103 99% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Cow, Human -
Product overview
Human CD80 ELISA Kit (B7-1) (ab256392) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD80 (B7-1) protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD80 (B7-1) with 31 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human CD80, also known as T-lymphocyte activation antigen CD80 and Activation B7-1 antigen, is a B7, type I membrane protein encoded by the gene CD80. CD80 is found on the membrane of dendritic cells, activated B and T cells, monocytes, and antigen-presenting cells (APC). CD80 functions as a receptor for CD28 and CTLA-4, which results in T and B-cell activation, proliferation, and differentiation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD80 (B7-1) Capture Antibody 1 x 600µl 10X Human CD80 (B7-1) Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CD80 (B7-1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
Database links
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD80 standard curve in Sample Diluent NS.The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CD80 in human cell culture supernatant samples.The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: Raji cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 1,255 pg/mL in Raji cell supernatant 100%.
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Interpolated concentrations of native CD80 in human serum and plasmas.The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, and plasma heparin 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 464 pg/mL in serum, 514 pg/mL in plasma (EDTA), 298 pg/mL in plasma (citrate), and 326 pg/mL in plasma (heparin).
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Serum from eight individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 780 pg/mL with a range of 572 – 1,371 pg/mL.
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Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (ab256392)To learn more about the advantages of recombinant antibodies see here.
