Human CD62E ELISA Set (without plates) (ab47348)
Overview
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Product name
Human CD62E ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
1 ng/ml - 32 ng/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s CD26E Human in vitro ELISA Set is designed for the quantitative measurement of CD26E in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The CD62E ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA set is intended to measure human CD62E in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant human CD62E.
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests CD62E Capture Antibody 1 x 0.5ml 2 x 0.5ml CD62E standard Yellow 5 vials 10 vials Detection biotinylated anti-CD62E antibody 1 x 0.55ml 2 x 0.55ml Chromogen TMB Substrate Solution 2 vials 4 vials Streptavidin-HRP 1 vial 2 vials -
Research areas
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Relevance
CD62E is expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by CD62E is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids). -
Cellular localization
Membrane; single-pass type I membrane protein -
Alternative names
- ELAM
- ELAM1
- ESEL
see all -
Database links
- Entrez Gene: 6401 Human
- SwissProt: P16581 Human
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests CD62E Capture Antibody 1 x 0.5ml 2 x 0.5ml CD62E standard Yellow 5 vials 10 vials Detection biotinylated anti-CD62E antibody 1 x 0.55ml 2 x 0.55ml Chromogen TMB Substrate Solution 2 vials 4 vials Streptavidin-HRP 1 vial 2 vials -
Research areas
-
Relevance
CD62E is expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by CD62E is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids). -
Cellular localization
Membrane; single-pass type I membrane protein -
Alternative names
- ELAM
- ELAM1
- ESEL
see all -
Database links
- Entrez Gene: 6401 Human
- SwissProt: P16581 Human
Images
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Human CD62E ELISA Set (ab47348)CD62E Standard curve ranging from 1 to 32 ng/mL