Human CD14 ELISA Kit (ab45523)
Key features and details
- Sensitivity: 6 ng/ml
- Range: 6.8 ng/ml - 220 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-CD14 2 x 0.3ml 1 x 0.3ml CD14 Microplate (12 x 8 well strips) 2 units 1 unit CD14 Standard (lyophilized) 4 vials 2 vials Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
Research areas
-
Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
-
Alternative names
- CD 14
- CD_antigen=CD14
- CD14
see all -
Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
Images
-
Sandwich ELISA -HuCD14 ELISA Kit
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted
-
Sandwich ELISA - HuCD14 Elisa Kithu CD14 measured in biologicals showing quantity (ng) per mL of tested sample
