Human Cathepsin D ELISA Kit (ab213470)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 ng/ml
- Range: 3.68 ng/ml - 125 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cathepsin D ELISA Kit
See all Cathepsin D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.6% Inter-assay Sample n Mean SD CV% Serum 3 5% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.4 ng/ml -
Range
3.68 ng/ml - 125 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 97 95% - 100% Milk 88 84% - 95% Urine 99 94% - 102% Serum 95 84% - 107% Cell culture media 93 89% - 95% Hep Plasma 96 81% - 108% EDTA Plasma 96 81% - 108% Cit plasma 80 76% - 85% Tissue Homogenate 121 111% - 134% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cathepsin D ELISA Kit (ab213470) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin D protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cathepsin D with 1.4 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Cathepsin D is a lysosomal aspartyl protease that is activated at low pH. Mutations in Cathepsin D have been linked to pathogensis of diseases including breast cancer and Alzheimer disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cathepsin D Capture Antibody 1 x 600µl 10X Human Cathepsin D Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Cathepsin D Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
Tissue specificity
Expressed in the aorta extrcellular space (at protein level). -
Involvement in disease
Ceroid lipofuscinosis, neuronal, 10 -
Sequence similarities
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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Alternative names
- CatD
- CATD_HUMAN
- Cathepsin D
see all -
Database links
- Entrez Gene: 1509 Human
- Omim: 116840 Human
- SwissProt: P07339 Human
- Unigene: 654447 Human
Images
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Other - Human Cathepsin D ELISA Kit (ab213470)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cathepsin D standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Cathepsin D standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cathepsin D in human serum and plasma samples.The concentrations of Cathepsin D were measured in duplicates, interpolated from the Cathepsin D standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 219.4 ng/mL in serum, 156.4 ng/mL in plasma (citrate), 245.6 ng/mL in plasma (heparin) and 281.9 ng/mL in plasma (EDTA).
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Interpolated concentrations of native Cathepsin D in human saliva, milk and HEPG2 cell culture supernatant samples.The concentrations of Cathepsin D were measured in duplicates, interpolated from the Cathepsin D standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 20%, milk 10%, and HEPG2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 618.7 ng/mL in saliva, 715.9 ng/mL in milk, and 120.3 ng/mL in HEPG2 cell culture supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 183 ng/mL with a range of 130 – 294 ng/mL.
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Interpolated concentrations of native Cathepsin D in Human Liver Homogenate Extract sample and sample based on a 12.5 µg/mL extract load.The concentrations of Cathepsin D were measured in duplicate and interpolated from the Cathepsin D standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration was determined to be 1088 ng/mL in Human Liver Homogenate Extract.
