Human Catalase ELISA Kit (ab277396)
Key features and details
- Sensitivity: 0.055 ng/ml
- Range: 0.051 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Catalase ELISA Kit
See all Catalase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.055 ng/ml -
Range
0.051 ng/ml - 12.5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102.3 97% - 106% Plasma 104.2 97% - 124% Cell culture media 78.59 72% - 84% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Catalase ELISA kit (ab277396) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Catalase in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human Catalase coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Catalase present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Catalase antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Catalase bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Note: Collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human Catalase Antibody 2 vials Anti-Human Catalase coated Microplate 1 unit Human Catalase standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
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Alternative names
- Cas1
- CAT
- CATA_HUMAN
see all -
Database links
- Entrez Gene: 847 Human
- Omim: 115500 Human
- SwissProt: P04040 Human
- Unigene: 502302 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.