Human Caspase 4 ELISA Kit (ab275098)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.7 pg/ml
- Range: 39 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Caspase 4 ELISA Kit
See all Caspase-4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.2% Inter-assay Sample n Mean SD CV% Extract 4 5% -
Sample type
Cell culture supernatant, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
6.7 pg/ml -
Range
39 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 112% - 117% Cell Lysate 118 115% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Caspase 4 ELISA kit (ab275098) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Caspase 4 protein in cell culture and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Caspase 4 with 6.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Caspase-4 also known as ICE and Ced-3 homolog 2, Mih1, and protease TX, is a protein in the family of cysteine proteases called caspases and is encoded by the gene CASP4. CASP4 is expressed as an inactive proenzyme comprised of a pro-domain and a large and small protease subunit. CASP4 is activated by cleavage of an internal Aspartic residue, thus generates a heterodimeric enzyme consisting of the two subunits which is capable of cleaving and activating its own precursor protein. Overexpression of CASP4 results in apoptosis. The antibodies used in this kit were immunized against regions in the large subunit (subunit 1).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Caspase 4 Capture Antibody 1 x 600µl 10X Human Caspase 4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Caspase 4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. -
Tissue specificity
Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. - Information by UniProt
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Alternative names
- Apoptotic cysteine protease Mih1/TX
- CASP-4
- CASP4
see all -
Database links
- Entrez Gene: 837 Human
- Omim: 602664 Human
- SwissProt: P49662 Human
- Unigene: 138378 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Caspase 4 standard curve in Sample Diluent NS.The Caspase 4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Caspase 4 in human THP-1 based on a 125 µg/mL extract load.The concentrations of Caspase 4 were measured in duplicate and interpolated from the Caspase 4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human Caspase 4 ELISA Kit (ab275098)To learn more about the advantages of recombinant antibodies see here.
