Human Caspase-1 ELISA Kit (ab219633)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.5 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Caspase-1 ELISA Kit
See all Caspase-1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell extract 3 4.4% Inter-assay Sample n Mean SD CV% cell extract 5 6.1% -
Sample type
Cell culture supernatant, Cell culture extracts, Tissue Extracts, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
5.5 pg/ml -
Range
39.06 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 95 89% - 99% Tissue Extracts 86 83% - 90% Cell culture media 103 101% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Caspase-1 ELISA Kit (ab219633) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Caspase-1 protein in tissue extracts, cell culture media, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Caspase-1 with 5.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Caspase-1 is a thiol protease that cleaves IL-1 beta precursor releasing the mature cytokine which is involved in a variety of inflammatory processes. Caspase-1 is part of the inflammasome complex. It is important for defense against pathogens. Caspase-1 also cleaves and activates sterol regulatory element binding proteins (SREBPs). Caspase-1 can also promote apoptosis as it is part of caspase cascade.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Caspase-1 Capture Antibody 1 x 600µl 10X Human Caspase-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Human Caspase-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. -
Tissue specificity
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- CASP-1
- CASP1
- CASP1_HUMAN
see all -
Database links
- Entrez Gene: 834 Human
- Omim: 147678 Human
- SwissProt: P29466 Human
- Unigene: 2490 Human
Images
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Other - Human Caspase-1 ELISA Kit (ab219633)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Caspase-1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Caspase-1 standard curve in 1X Cell Extraction Buffer PTR.Example of human Caspase-1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Caspase-1 in human LPS treated (8 hours), mock treated (8 hours), and untreated THP-1 cell extract samples based on a 50 µg/mL extract load.The concentrations of Caspase-1 were measured in duplicate and interpolated from the Caspase-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 1497 pg/mL in LPS treated (8 hours) THP-1 cell extract, 1586 pg/mL in mock treated (8 hours) THP-1 cell extract, and 1735 pg/mL in untreated THP-1 cell extract.
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Interpolated concentrations of native Caspase-1 in human thyroid tissue extract based on a 500 µg/mL extract load and BxPC-3 cell extract based on a 250 µg/mL extract load.The concentrations of Caspase-1 were measured in duplicate and interpolated from the Caspase-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 128.7 pg/mL in thyroid tissue extract and 2279 pg/mL in BxPC-3 cell extract.
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Interpolated concentrations of native Caspase-1 in human cell culture supernatant samples.Undiluted samples are as follows: THP-1 LPS neat, THP-1 mock neat, PBMC PHA-M neat, PBMC mock neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 549 pg/mL in LPS treated (8 hours) THP-1 cell culture supernatant, 41.8 pg/mL in mock treated (8 hours) THP-1 cell culture supernatant, 204 pg/mL PHA-M treated (2 days) PBMC cell culture supernatant, and 57.3 pg/mL in mock treated (2 days) PBMC cell culture supernatant.
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Comparison of Caspase-1 in unstimulated and LPS stimulated THP-1 cell supernatants and unstimulated and PHA-M stimulated PBMC cell supernatants.Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 2 days. The concentrations of Caspase-1 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the Caspase-1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean Caspase-1 concentration was determined to be 549 pg/mL in LPS stimulated THP-1 cell supernatant, 41.8 pg/mL in unstimulated THP-1 cell supernatant, 204 pg/mL in PHA-M stimulated PBMC cell supernatant, 57.3 pg/mL in unstimulated PBMC supernatant, and undetectable in media (not shown).
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Sandwich ELISA - Human Caspase-1 ELISA Kit (ab219633)To learn more about the advantages of recombinant antibodies see here.
