Human Carbonic Anhydrase 6 (CA6) ELISA Kit (ab275899)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.12 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cit plasma, Milk, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Carbonic Anhydrase 6 (CA6) ELISA Kit
See all Carbonic Anhydrase 6/CA6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.9% Inter-assay Sample n Mean SD CV% Serum 3 4.3% -
Sample type
Saliva, Milk, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.12 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 95 93% - 96% Milk 102 102% - 102% Serum 104 100% - 109% Tissue Extracts 95 87% - 100% Cit plasma 109 106% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Carbonic anhydrase 6 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Carbonic anhydrase 6 protein in human serum, plasma, milk, saliva, and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Carbonic anhydrase 6 with 2.12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Carbonic anhydrase 6 (CA6), encoded by CA6 gene, is a zinc metalloenzyme. More specifically, an extracellular secretory isoform of the CA family that catalyzes the reversible hydration-dehydration of CO2 and HCO3-1: CO2 + H2O + + HCO3- and found in all tissue and cell types. CA6 main presence is found in saliva, though its function in saliva is unknown.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Carbonic Anhydrase 6 (CA6) Capture Antibody 1 x 600µl 10X Human Carbonic Anhydrase 6 (CA6) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Carbonic Anhydrase 6 (CA6) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Reversible hydration of carbon dioxide. Its role in saliva is unknown. -
Tissue specificity
Major constituent of saliva. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CA VI
- CA-VI
- CA6
see all -
Database links
- Entrez Gene: 765 Human
- Omim: 114780 Human
- SwissProt: P23280 Human
- Unigene: 100322 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Carbonic Anhydrase 6 standard curve in Sample Diluent NS.The Carbonic Anhydrase 6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Carbonic Anhydrase 6 standard curve in 1X Cell Extraction Buffer PTR.The Carbonic Anhydrase 6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Carbonic Anhydrase 6 in human serum, plasma, milk, and saliva samples.The concentrations of Carbonic Anhydrase 6 were measured in duplicates, interpolated from the Carbonic Anhydrase 6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100, plasma (citrate) 1:100, milk 1:10,000, and saliva 1:25,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Carbonic Anhydrase 6 concentration was determined to be 87.8 ng/mL in serum, 97.4 ng/mL in plasma (citrate), 6,159 ng/mL in milk, and 10,255 ng/mL in saliva.
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Interpolated concentrations of native Carbonic Anhydrase 6 in human kidney sample based on a 500 µg/mL extract load.The concentrations of Carbonic Anhydrase 6 were measured in duplicate and interpolated from the Carbonic Anhydrase 6 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Carbonic Anhydrase 6 concentration was determined to be 32.4 pg/mL in kidney sample.
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Sandwich ELISA - Human Carbonic Anhydrase 6 (CA6) ELISA Kit (ab275899)To learn more about the advantages of recombinant antibodies see here.
