Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human C4b Antibody Pair - BSA and Azide free (Complement C4-B) (ab253490)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human C4b Antibody Pair - BSA and Azide free (Complement C4-B)
    See all C4b kits

  • Assay type

    ELISA set

  • Range

    140.625 pg/ml - 9000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    The Antibody Pair can be used to quantify Human C4b. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277717), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human C4b (Complement C4-B) Capture Antibody (unconjugated) 1 x 100µg
    Human C4b (Complement C4-B) Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.

  • Post-translational
    modifications

    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Basic complement C4
    • C2B5
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
    • C4b
    • C4B1
    • C4B2
    • C4B3
    • CO4B_HUMAN
    • Complement C4 gamma chain
    • Complement component 4B
    • CPAMD3
    see all

  • Database links

    see all

Images

  • Example of Human C4b (Complement C4-B) standard curve.
    Example of Human C4b (Complement C4-B) standard curve.

    Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277717), which uses the same antibody pair. Note that the reference range provided for the pair is an estimation based on the performance of the related kit.

  • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253490)
    Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253490)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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