Human BMP9 ELISA Kit (ab267648)
Key features and details
- Sensitivity: 32 pg/ml
- Range: 32.77 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BMP9 ELISA Kit
See all BMP9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
32 pg/ml -
Range
32.77 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 98.34 84% - 126% Plasma 87.04 68% - 137% Cell culture media 92.95 84% - 100% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human BMP9 ELISA Kit is designed for the quantitative determination of BMP9 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human BMP9 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin A, aFGF, Amphiregulin, bFGF, BMP-4, E-Selectin, ICAM-1, IGF-1, IL-1 alpha, IL-1 beta, IL-6, IL8, IL-11, IL-17A, MCP-1, M-CSF, MIP-1 alpha, MMP-2, MMP-9, MMP-13, Osteoactivin, PCadherin, RANK, SDF-1 alpha, Shh-N, TGF beta 1, TGF beta 2, TNF alpha, VCAM-1, VECadherin.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human BMP9 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human BMP9 Detection Antibody 2 vials Human BMP9 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. They are regulators of cell growth and differentiation. BMP9 has a role in the maturation of BFCN (basal forebrain cholinergic neurons) also inducing and maintaining the ability of these cells to respond to acetylcholine; BFCN are important for the processes of learning, memory and attention. BMP9 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in hepatocytes and can regulate iron metabolism. The physiological receptor of BMP9 is thought to be activin receptor-like kinase 1, ALK1 (also called ACVRL1), an endothelial-specific type I receptor of the TGF-beta receptor family. BMP9 is one of the most potent BMPs to induce orthotopic bone formation in vivo. BMP3, a blocker of most BMPs seems not to affect BMP9. -
Cellular localization
Secreted -
Alternative names
- BMP 9
- Bone morphogenetic protein 9
- GDF 2
see all -
Database links
- Entrez Gene: 2658 Human
- Omim: 605120 Human
- SwissProt: Q9UK05 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.
