Human beta 2 Microglobulin ELISA Kit (ab108885)
Key features and details
- Sensitivity: 33 pg/ml
- Range: 0.19 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human beta 2 Microglobulin ELISA Kit
See all beta 2 Microglobulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.3% Inter-assay Sample n Mean SD CV% Overall 10.3% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 33 pg/ml -
Range
0.19 ng/ml - 12.5 ng/ml -
Recovery
97 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s beta 2 Microglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of beta 2 Microglobulin levels in plasma, serum, milk, saliva, urine, CSF and cell culture supernatants.
A beta 2 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a beta 2 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of beta 2 Microglobulin captured in plate.
Get results in 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Biotinylated beta 2 Microglobulin Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml beta 2 Microglobulin Microplate (12 x 8 well strips) 1 unit beta 2 Microglobulin Standard 2 vials Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Alternative names
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
Images
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Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (mU) per mL of tested sample. Human serum and plasma samples diluted 1000-8000 fold. Human milk diluted 4000-32000 fold. Human urine diluted 100-200 fold. Human saliva diluted 400-1600 fold. Mouse and rat samples diluted 10 fold. HepG2 supernatant diluted 1000 fold.
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Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)Standard curve: mean of duplicates (+/- SD) with background reads subtracted -
Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample
