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Human beta 2 Microglobulin ELISA Kit (ab108885)

Key features and details

  • Sensitivity: 33 pg/ml
  • Range: 0.19 ng/ml - 12.5 ng/ml
  • Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human beta 2 Microglobulin ELISA Kit
    See all beta 2 Microglobulin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.3%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 33 pg/ml

  • Range

    0.19 ng/ml - 12.5 ng/ml

  • Recovery

    97 %

  • Assay time

    4h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s beta 2 Microglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of beta 2 Microglobulin levels in plasma, serum, milk, saliva, urine, CSF and cell culture supernatants.


    A beta 2 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a beta 2 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of beta 2 Microglobulin captured in plate.


    Get results in 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Biotinylated beta 2 Microglobulin Antibody 1 x 60µl
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    beta 2 Microglobulin Microplate (12 x 8 well strips) 1 unit
    beta 2 Microglobulin Standard 2 vials
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml

  • Research areas

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.

  • Cellular localization

    Secreted. Detected in serum and urine.
  • Information by UniProt

  • Alternative names

    • B2M
    • B2MG_HUMAN
    • Beta 2 microglobin
    • Beta 2 microglobulin
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Beta chain of MHC class I molecules
    • Beta-2-microglobulin form pI 5.3
    • CDABP0092
    • Hdcma22p
    • IMD43
    see all

  • Database links

    Images

    • Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)
      Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)

      Standard curve: mean of duplicates (+/- SD) with background reads subtracted

    • Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)
      Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)

      Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (mU) per mL of tested sample. Human serum and plasma samples diluted 1000-8000 fold. Human milk diluted 4000-32000 fold. Human urine diluted 100-200 fold. Human saliva diluted 400-1600 fold. Mouse and rat samples diluted 10 fold. HepG2 supernatant diluted 1000 fold.

    • Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)
      Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)
      Standard curve: mean of duplicates (+/- SD) with background reads subtracted
    • Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)
      Sandwich ELISA - beta 2 Microglobulin Human ELISA Kit (ab108885)
      Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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