Human BAG3 ELISA Kit (ab227900)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.7 pg/well
- Range: 39.1 pg/ml - 2500 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BAG3 ELISA Kit
See all Bag3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HEK-293T 5 5.2% Inter-assay Sample n Mean SD CV% HEK-293T 3 2.5% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
11.7 pg/well -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 97 93% - 99% Tissue Extracts 104 103% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human BAG3 ELISA Kit (ab227900) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BAG3 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BAG3 with 11.7 pg/well sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BAG3 Capture Antibody 1 x 600µl 10X Human BAG3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human BAG3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. -
Involvement in disease
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. -
Sequence similarities
Contains 1 BAG domain.
Contains 2 WW domains. - Information by UniProt
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Alternative names
- BAG 3
- BAG family molecular chaperone regulator 3
- BAG-3
see all -
Database links
- Entrez Gene: 9531 Human
- Omim: 603883 Human
- SwissProt: O95817 Human
- Unigene: 523309 Human
Images
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Other - Human BAG3 ELISA Kit (ab227900)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of humanBag3 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Bag3 in HEK293T cell extract based on a 100 µg/mL extract load and human heart tissue extract based on a 150 µg/mL extract loadThe concentrations of Bag3 were measured in duplicate and interpolated from the Bag3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Bag3 concentration was determined to be 2497 pg/mL in HEK293T cell extract and 2743 pg/mL in human heart tissue extract.
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Sandwich ELISA - Human BAG3 ELISA Kit (ab227900)To learn more about the advantages of recombinant antibodies see here.
