Microplate wells are pre-coated with anti-human Artemin-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human Artemin molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human Artemin-specific monoclonal detection antibodies bind to the same epitope on another molecule of dimeric human Artemin. After washing, the ready-to-use HRP substrate (TMB) is added to the wells. The intensity of the color produced is directly proportional to the amount of human Artemin in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.