Human Anti-ENA Screen ELISA Kit (ab178619)
Key features and details
- Sensitivity: 3.68 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-ENA Screen ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 32 Inter-assay Sample n Mean SD CV% Overall 2 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
3.68 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-ENA Screen Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate semi-quantitative measurement of IgG class antibodies against extractable nuclear antigens: Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52kDa and 60kDa), SS-B (La), Jo-1, in Human serum or plasma.
A 96-well plate has been precoated with ENA antigens to bind cognate antibodies. Controls, standards or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized ENA-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of ENA IgG sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 1 x 50ml Cover Foil 1 unit ENA Anti-IgG HRP Conjugate 1 x 15ml ENA Coated Microplate (12 x 8 wells) 1 unit ENA Negative Control 1 x 1.2ml ENA Positive Control 1 x 1.2ml ENA Standard 1 x 1.2ml IgG Sample Diluent 1 x 100ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
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Relevance
Extractable nuclear antigens (ENA): Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52kDa and 60kDa), SS-B (La), Jo-1, in Human serum or plasma. ENA is a grouping of antibodies often used to screen for mixed connective tissue disease (MCTD), Sjögren's syndrome and systemic lupus erythematosus and commonly is composed of four tests.
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.