Human Anti-Candida albicans IgM ELISA Kit (ab108716)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Candida albicans IgM ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos.Serum 6 4.5% Inter-assay Sample n Mean SD CV% Pos.Serum 38 5.1% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Candida albicans IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Candida albicans in Human serum or plasma.
A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgM sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Candida albicans (IgM) Coated Microplate (12 x 8 wells) 1 unit Candida albicans anti-IgM HRP Conjugate Red Bottle, Black Cap 1 x 20ml Candida albicans IgM Cut-off Control Yellow Bottle, Green Cap 1 x 3ml Candida albicans IgM Negative Control Yellow Bottle, Blue Cap 1 x 2ml Candida albicans IgM Positive Control Yellow Bottle, Red Cap 1 x 2ml Foil Cover 1 unit IgM Sample Diluent Green Bottle, White Cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip Holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
