Human Annexin A2 ELISA Kit (ab264612)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.86 ng/ml
- Range: 8.8 ng/ml - 100 ng/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Annexin A2 ELISA Kit
See all Annexin-2/ANXA2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.6% Inter-assay Sample n Mean SD CV% Extract 4 7.1% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
1.86 ng/ml -
Range
8.8 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 97 94% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Annexin A2 ELISA Kit (ab264612) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin A2 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Annexin A2 with 1.86 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Annexin A2 is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism, but also competes with LDLR for binding with PCSK9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Annexin A2 Capture Antibody 1 x 600µl 10X Human Annexin A2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Annexin A2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
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Alternative names
- Annexin A2
- Annexin II
- Annexin II, heavy chain
see all -
Database links
- Entrez Gene: 302 Human
- Omim: 151740 Human
- SwissProt: P07355 Human
- Unigene: 511605 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Annexin A2 standard curve in Sample Diluent 1X Cell Extract Buffer PTR.The Annexin A2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Annexin A2 in human A431 cell extract based on a 4.4 µg/mL extract load.The concentrations of Annexin A2 were measured in duplicate and interpolated from the Annexin A2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human Annexin A2 ELISA Kit (ab264612)To learn more about the advantages of recombinant antibodies see here.
