Human AMICA ELISA Kit (JAML) (ab267597)
Key features and details
- Sensitivity: 0.41 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human AMICA ELISA Kit (JAML) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall Inter-assay Sample n Mean SD CV% overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.41 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 116.3 97% - 137% Plasma 111.3 87% - 133% Cell culture media 88.7 86% - 93% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human AMICA ELISA Kit is designed for the quantitative determination of AMICA in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human AMICA coated on a 96-well plate. Standards and samples are pipetted into the wells and AMICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AMICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AMICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human aFGF, Angiopoietin-4, BAFF, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, DLL1, Fetuin A, FOLR1, GASP-1, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRANCE, Troponin I, uPA, WISP-1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human AMICA coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human AMICA Detection Antibody 2 vials Human AMICA Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
May function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells. -
Tissue specificity
Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Domain
The Ig-like V-type domain 2 mediates interaction with CXADR. -
Cellular localization
Cell junction. Cell membrane. Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN. - Information by UniProt
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Alternative names
- adhesion molecule AMICA
- Adhesion molecule interacting with CXADR antigen 1
- adhesion molecule interacts with CXADR antigen 1
see all -
Database links
- Entrez Gene: 120425 Human
- Omim: 609770 Human
- SwissProt: Q86YT9 Human
- Unigene: 16291 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.