Human AG-2 ELISA Kit (ab240687)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.7 pg/ml
- Range: 11.7 pg/ml - 750 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human AG-2 ELISA Kit
See all Anterior Gradient 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 8 3.1% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
3.7 pg/ml -
Range
11.7 pg/ml - 750 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 97 % - % Tissue Extracts 99 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human AG-2 ELISA Kit (ab240687) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AG-2 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human AG-2 with 3.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human AG-2 Capture Antibody 1 x 600µl 10X Human AG-2 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human AG-2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. -
Tissue specificity
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. -
Sequence similarities
Belongs to the AGR family. -
Cellular localization
Secreted. Endoplasmic reticulum. - Information by UniProt
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Alternative names
- AG 2
- AG 2 protein
- AG-2
see all -
Database links
- Entrez Gene: 10551 Human
- Omim: 606358 Human
- SwissProt: O95994 Human
- Unigene: 530009 Human
Images
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Other - Human AG-2 ELISA Kit (ab240687)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human AG-2 standard curve in 1X Cell Extraction Buffer PTR.The AG-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native AG-2 in human PC3 cell extract based on a 1.3 µg/mL extract load and in human fetal small intestine tissue homogenate extract based on a 12.5 µg/mL extract load.The concentrations of AG-2 were measured in duplicate and interpolated from the AG-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AG-2 concentration was determined to be 827 pg/mL in PC3 cell extract and 547 pg/mL in human fetal small intestine tissue homogenate extract. -
Sandwich ELISA - Human AG-2 ELISA Kit (ab240687)To learn more about the advantages of recombinant antibodies see here.
