Human ADAM9 ELISA Kit (ab193690)
Key features and details
- Sensitivity: 45 pg/ml
- Range: 45 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ADAM9 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
45 pg/ml -
Range
45 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96.06 88% - 104% Plasma 91.5 81% - 101% Cell culture media 122.2 114% - 130% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s ADAM9 Human ELISA Kit (ab193690) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ADAM9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human ADAM9 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures ADAM9 present in the samples. The wells are washed and biotinylated anti-Human ADAM9 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of ADAM9 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Detection Antibody ADAM9 (biotinylated anti-Human ADAM9) 2 vials Human ADAM9 Standards (lyophilized) 2 vials Pre-coated Human ADAM9 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Function
Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. -
Tissue specificity
Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. -
Involvement in disease
Cone-rod dystrophy 9 -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ADAM 9
- ADAM metallopeptidase domain 9
- Adam9
see all -
Database links
- Entrez Gene: 8754 Human
- Omim: 602713 Human
- SwissProt: Q13443 Human
- Unigene: 591852 Human
Images
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Standard Curve.
Standard Curve in Assay Diluent A using ab193690 ADAM9 Human ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Standard Curve.Standard Curve in Assay Diluent B using ab193690 ADAM9 Human ELISA Kit.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.