Human ACY-1 ELISA (ab277463)
Key features and details
- Sensitivity: 0.19 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ACY-1 ELISA -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.19 ng/ml -
Range
0.164 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 86.56 72% - 120% Plasma 87.45 71% - 108% Cell culture media 90.4 72% - 121% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human ACY-1 ELISA Kit (ab277463) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ACY-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human ACY-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ACY-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ACY-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ACY-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human ACY-1 Antibody 2 vials Anti-Human ACY-1 coated Microplate 1 unit Human ACY-1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). -
Tissue specificity
Expression is highest in kidney, strong in brain and weaker in placenta and spleen. -
Involvement in disease
Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D) [MIM:609924]. ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. -
Sequence similarities
Belongs to the peptidase M20A family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- ACY 1
- ACY-1
- Acy1
see all -
Database links
- Entrez Gene: 95 Human
- Omim: 104620 Human
- SwissProt: Q03154 Human
- Unigene: 334707 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.