HsMyoD1 ChIP probe (ab83599)
Overview
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Product name
HsMyoD1 ChIP probe -
General notes
Accession number: NM_002478.The HsMYOD1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. MYOD1 (Myoblast determination protein 1) is involved in muscle development. The primers and probe are located in exon 1. In most cell types MYOD1 is enriched with histone modifications associated with silencing (e.g. Histone H3 K27 di methylation, see image with ab24684). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441).
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Tested applications
Suitable for: Real Time PCRmore details
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: ddH20 -
Concentration information loading... -
Research areas
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- bHLHc1
- Class C basic helix-loop-helix protein 1
- MYF 3
see all
