HRP Anti-PD-L1 antibody [28-8] (ab209961)
![HRP Anti-PD-L1 antibody [28-8] (ab209961)](https://www.abcam.com/ps/products/209/ab209961/Images/ab209961-258338-anti-pd-l1-antibody-28-8-hrp-western-blot.jpg "HRP Anti-PD-L1 antibody [28-8] (ab209961)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [28-8] to PD-L1
- Suitable for: WB
- Reacts with: Chinese hamster
- Conjugation: HRP
Overview
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Product name
HRP Anti-PD-L1 antibody [28-8]
See all PD-L1 primary antibodies -
Description
HRP Rabbit monoclonal [28-8] to PD-L1 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Chinese hamster
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Immunogen
Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
Database link: Q9NZQ7 -
Positive control
- WB: CHO-PD-L1 whole cell lysate.
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General notes
Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565)
Alternative versions available:
Anti-PD-L1 antibody [28-8] (ab205921) - Knockout validatedOur RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
28-8 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-PD-L1 antibody [28-8] (ab209961)All lanes : HRP Anti-PD-L1 antibody [28-8] (ab209961) at 1/1000 dilution
Lane 1 : CHO-S
Lane 2 : CHO-PD-L1
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209961 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-PD-L1 antibody [28-8] (ab209961)](https://www.abcam.com/ps/products/209/ab209961/Images/ab209961-2-benefits-of-recombinant-antibodies.png)
HRP Anti-PD-L1 antibody [28-8] (ab209961)
