HRP Anti-CLPX antibody [EP8772] (ab203694)
![HRP Anti-CLPX antibody [EP8772] (ab203694)](https://www.abcam.com/ps/products/203/ab203694/Images/ab203694-252489-anti-clpx-antibody-ep8772-hrp-western-blot.jpg "HRP Anti-CLPX antibody [EP8772] (ab203694)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP8772] to CLPX
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-CLPX antibody [EP8772]
See all CLPX primary antibodies -
Description
HRP Rabbit monoclonal [EP8772] to CLPX -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human CLPX. The exact sequence is proprietary.
Database link: O76031 -
Positive control
- WB: HT-1080 and Saos-2 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP8772 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-CLPX antibody [EP8772] (ab203694)All lanes : HRP Anti-CLPX antibody [EP8772] (ab203694) at 1/5000 dilution
Lane 1 : HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203694 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-CLPX antibody [EP8772] (ab203694)](https://www.abcam.com/ps/products/203/ab203694/Images/ab203694-3-benefits-of-recombinant-antibodies.png)
HRP Anti-CLPX antibody [EP8772] (ab203694)
