HRP Anti-CD68 antibody [FA-11] (ab216526)
![HRP Anti-CD68 antibody [FA-11] (ab216526)](https://www.abcam.com/ps/products/216/ab216526/Images/ab216526-284029-anti-cd68-antibody-fa-11-hrp-western-blot.jpg "HRP Anti-CD68 antibody [FA-11] (ab216526)")
Key features and details
- HRP Rat monoclonal [FA-11] to CD68
- Suitable for: WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-CD68 antibody [FA-11]
See all CD68 primary antibodies -
Description
HRP Rat monoclonal [FA-11] to CD68 -
Host species
Rat -
Conjugation
HRP -
Specificity
Detects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Tissue, cells or virus corresponding to Mouse CD68.
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Positive control
- Raw264.7
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
FA-11 -
Isotype
IgG2a -
Research areas
Images
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Western blot - HRP Anti-CD68 antibody [FA-11] (ab216526)All lanes : HRP Anti-CD68 antibody [FA-11] (ab216526) at 1/5000 dilution
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : RAW 264.7 LPS treated (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Additional bands at: 100 kDa (possible glycosylated form)
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab216526 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
