HRP Anti-CaMKII antibody [6G9] (ab197500)
![HRP Anti-CaMKII antibody [6G9] (ab197500)](https://www.abcam.com/ps/products/197/ab197500/Images/ab197500-238345-anti-camkii-antibody-6g9-hrp-western-blot.jpg "HRP Anti-CaMKII antibody [6G9] (ab197500)")
Key features and details
- HRP Mouse monoclonal [6G9] to CaMKII
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-CaMKII antibody [6G9]
See all CaMKII primary antibodies -
Description
HRP Mouse monoclonal [6G9] to CaMKII -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Full length native protein (purified) corresponding to Rat CaMKII.
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Positive control
- WB: Rat, mouse and human brain tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
6G9 -
Isotype
IgG1 -
Research areas
Images
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Western blot - HRP Anti-CaMKII antibody [6G9] (ab197500)All lanes : HRP Anti-CaMKII antibody [6G9] (ab197500) at 1/5000 dilution
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197500 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
