HRP Anti-Blue Ladder antibody [2D2-F11] (ab231760)
![HRP Anti-Blue Ladder antibody [2D2-F11] (ab231760)](https://www.abcam.com/ps/products/231/ab231760/Images/ab231760-308452-WB183ab231760AP3415134GDB.jpg "HRP Anti-Blue Ladder antibody [2D2-F11] (ab231760)")
Key features and details
- HRP Mouse monoclonal [2D2-F11] to Blue Ladder
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG1
Overview
-
Product name
HRP Anti-Blue Ladder antibody [2D2-F11] -
Description
HRP Mouse monoclonal [2D2-F11] to Blue Ladder -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to Blue Ladder. Remazol brilliant blue R stained proteins.
-
General notes
Ab231760 detects blue pre-stained protein markers (specifically Remazol brilliant blue R dye stained proteins). It allows the visualisation of pre-stained protein ladders in combination with the bands of interest without needing to merge images or manually mark film (PubMed ID: 27531616). The antibody is conjugated to HRP to permit direct visualisation by chemiluminescence.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2D2-F11 -
Isotype
IgG1 -
Light chain type
kappa
Images
-
Western blot - HRP Anti-Blue Ladder antibody [2D2-F11] (ab231760)All lanes : HRP Anti-Blue Ladder antibody [2D2-F11] (ab231760) at 1/5000 dilution
Lane 1 : Marker Chameleon
Lane 2 : Thermo Scientific Pageruler Plus
Lane 3 : All Blue BioRad
Lysates/proteins at 1 µl per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab231760 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.