Antibodies, Proteins, Kits and Reagents for Life Science

HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EP1569Y] to beta III Tubulin
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human
Conjugation: HRP

Product name

HRP Anti-beta III Tubulin antibody [EP1569Y]
See all beta III Tubulin primary antibodies
Description

HRP Rabbit monoclonal [EP1569Y] to beta III Tubulin
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and PC12 whole cell lysates. Mouse brain, mouse spinal cord, rat brain and rat spinal cord tissue lysates.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1569Y
Isotype

IgG
Research areas

Western blot - HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574)

Lane 1 : HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574) at 20 µg
Lane 2 : HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574) at 1/5000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TUBB3 (beta III Tubulin) knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 2 minutes

ab190574 was shown to specifically react with beta III Tubulin in wild-type HAP1 cells as signal was lost in TUBB3 (beta III Tubulin) knockout cells. Wild-type and TUBB3 (beta III Tubulin) knockout samples were subjected to SDS-PAGE. Ab190574 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Western blot - HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574)

All lanes : HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574) at 1/3000 dilution

Lane 1 : HeLa whole cell lysate (ab150035)
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Lysates/proteins at 20 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 50 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190574 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Western blot - HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574)

All lanes : HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574) at 1/10000 dilution

Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Spinal Cord (Rat) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190574 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-beta III Tubulin antibody [EP1569Y] (ab190574)

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