HRP Anti-beta III Tubulin antibody [2G10] (ab196638)
![HRP Anti-beta III Tubulin antibody [2G10] (ab196638)](https://www.abcam.com/ps/products/196/ab196638/Images/ab196638-312604-anti-beta-iii-tubulin-antibody-2g10-hrp-western-blot.jpg "HRP Anti-beta III Tubulin antibody [2G10] (ab196638)")
Key features and details
- HRP Mouse monoclonal [2G10] to beta III Tubulin
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-beta III Tubulin antibody [2G10]
See all beta III Tubulin primary antibodies -
Description
HRP Mouse monoclonal [2G10] to beta III Tubulin -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Common marmoset
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Immunogen
Tissue, cells or virus corresponding to Rat beta III Tubulin.
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Positive control
- WB: Human, mouse and rat brain tissue lysates. IHC: Human cerebellum tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
2G10 -
Isotype
IgG2a -
Research areas
Images
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Western blot - HRP Anti-beta III Tubulin antibody [2G10] (ab196638)All lanes : HRP Anti-beta III Tubulin antibody [2G10] (ab196638) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TUBB3 (beta III Tubulin) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 50 kDa
Exposure time: 2 minutesab196638 was shown to specifically react with beta III Tubulin in wild-type HAP1 cells as signal was lost in TUBB3 (beta III Tubulin) knockout cells. Wild-type and TUBB3 (beta III Tubulin) knockout samples were subjected to SDS-PAGE. Ab196638 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-beta III Tubulin antibody [2G10] (ab196638)](https://www.abcam.com/ps/products/196/ab196638/Images/ab196638-238286-anti-beta-iii-tubulin-antibody-2g10-hrp-western-blot.jpg)
Western blot - HRP Anti-beta III Tubulin antibody [2G10] (ab196638)All lanes : HRP Anti-beta III Tubulin antibody [2G10] (ab196638) at 1/5000 dilution
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196638 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta III Tubulin antibody [2G10] (ab196638)](https://www.abcam.com/ps/products/196/ab196638/Images/ab196638-237364-anti-beta-iii-tubulin-antibody-2g10-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta III Tubulin antibody [2G10] (ab196638)IHC image of beta III Tubulin staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196638 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta III Tubulin antibody [2G10] (ab196638)](https://www.abcam.com/ps/products/196/ab196638/Images/ab196638-237363-anti-beta-iii-tubulin-antibody-2g10-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-beta III Tubulin antibody [2G10] (ab196638)IHC image of beta III Tubulin staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196638 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
