Hev b5 ELISA kit (ab215423)
Key features and details
- Sensitivity: 0.5 µg/L
- Range: 5 µg/L - 100 µg/L
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Hev b5 ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Spike (low) 16 21µg/L 0.9 4.4% Spike (med) 16 31µg/L 0.8 2.5% Spike (high) 16 76µg/L 3.8 5.1% Inter-assay Sample n Mean SD CV% Spike (low) 5 5.7µg/L 0.3 5.2% Spike (med) 5 24µg/L 0.6 2.6% Spike (high) 5 86µg/L 4.7 5.4% -
Assay type
Sandwich (quantitative) -
Sensitivity
0.5 µg/L -
Range
5 µg/L - 100 µg/L -
Recovery
Sample specific recovery Sample type Average % Range Spike 99 0µg/L - 100µg/L -
Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Product overview
The Hev b5 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab215423) is designed for the quantitative measurement of of Hev b5 in natural rubber latex products.
This kit is based on the immunoenzymetric assay technique. Microplate wells are coated with anti-Hev b5 specific monoclonal capture antibody that during the first incubation binds Hev b5 molecules from the sample extract. After incubation, unbound material is removed by washing the wells. During the second incubation, horseradish peroxidase (HRP) labelled Hev b5-specific monoclonal detection antibody binds to a different epitope of Hev b5 molecules. As such, the two antibodies are a matched pair that forms a “sandwich” around the target Hev b5 allergen. After washing, HRP substrate is added and the intensity of the colour produced is directly proportional to the Hev b5 concentration of the sample.
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Notes
Products containing natural rubber latex (NRL) from the rubber tree Hevea brasiliensis are widely used due to the economical price and advantageous processing properties of natural rubber, although adverse reactions against a number of allergenic proteins contained in the NRL are well known and documented.
Latex allergens are proteins or polypeptides eluting from the manufactured products upon contact with skin, mucous membranes or other tissues. According to the current allergen nomenclature system maintained by the International Union of Immunological Societies (IUIS) under the WHO, thirteen latex allergens, which have been characterized at the primary structure level and are contained in the official allergen list, are named as Hev b1, Hev b 2, Hev b 3, Hev b 4, Hev b 5, Hev b 6 (6.01, 6.02, 6.03), Hev b 7, Hev b 8, Hev b 9, Hev b 10, Hev b 11, Hev b 12 and Hev b 13. Not all previously listed allergens are able to come through the production process. At present, four of these allergens (Hev b1, Hev b 3, Hev b 5 and Hev b 6.02) have been mostly detected in manufactured latex products.
Hev b5 ELISA (ab215423) kit is the first commercial quantitative test to measure Hev b5 immunologically in NRL products. By the use of specific monoclonal antibodies, sensitivity and specificity stay high even in the presence of other proteins or chemical substances derived from the manufacturing process of NRL products.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests anti-Hev b5 coated microplate (12 x 8 wells) 1 unit Hev b 5 Assay Buffer 1 x 15ml Hev b 5 Calibrator A (0 µg/L) 1 x 500µl Hev b 5 Calibrator B (5 µg/L) 1 x 500µl Hev b 5 Calibrator C (10 µg/L) 1 x 500µl Hev b 5 Calibrator D (25 µg/L) 1 x 500µl Hev b 5 Calibrator E (50 µg/L) 1 x 500µl Hev b 5 Calibrator F (100 µg/L) 1 x 500µl Hev b 5 Control 1 x 500µl Hev b 5 Enzyme Conjugate 1 x 15ml HRP Substrate Solution 1 x 15ml PBS Wash Concentrate 1 x 50ml Stopping Solution 1 x 15ml -
Research areas
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Relevance
Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02. -
Alternative names
- Hev b 5
- Major latex allergen
Images
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Hev b5 ELISA kit (ab215423) typical standard curve.